AAV Stability & Titer In Viral Vector Manufacturing

With Fluorescence Membrane Microscopy, getting to the root of AAV stability issues is quick and easy. Our innovative technology only requires 5 µL of material for ultra-fast insights, so you can gain clarity into gene therapy formulations sooner.

Characterize AAV Stability in Late Discovery

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Why Use Aura to Monitor DNA Leakage-Based SVP Aggregation?

Because you can achieve so much more insight with much less material:
  • Achieve more accurate results with minimal amounts of sample (as little as 5 μL per test)
  • ID SYBR-labeled DNA aggregates from unstable AAV vectors
  • Detect and quantitate particles not measured by DLS or SEC
  • Determine root cause for unstable gene therapies
  • 96-well format for high-throughput testing
  • Gather comprehensive data and insight into aggregate particles – size, morphology, counts, distribution
  • Get insights quickly with rapid analysis time of 1 minute per sample
  • Benefit from a wide working range: measure particles from 1 μm to 5 mm with high reproducibility
  • Analyze particles without the interference of buffer or matrix for higher sensitivity
  • 21 CFR Part 11 software available

Identify Root Cause of Aggregates at Low Volume

Detect DNA leakage from stressed AAV empty or full capsids and to determine the cause of AAV aggregation.

FMM Empowers High-Throughput Subvisible Particle Characterization

Scatterplot data for (a) full AAV capsids and (b) empty AAV capsids. The y-axis represents fluorescence intensity in the SYBR Gold channel, and the x-axis represents the equivalent circular diameter (ECD) for the individual particle. The full AAV capsid (left) had 16–fold as many particles and almost 3x stronger average fluorescence intensity than the empty capsid sample (right).