Fluorescence Membrane Microscopy – Get Smarter, Faster Results
Fluorescence Membrane Microscopy (FMM) pushes the limits of particle analysis with its ultra-fast sampling and 100% efficiency, allowing for detailed insights into particle characterization and identification. When combined with Backgrounded Membrane Imaging (BMI), you get a powerful screening tool for faster, smarter results.
Know What’s In Your Protein, Cell and Gene Therapies
Characterizing biopharmaceutical products is no easy task, and traditional "counting techniques" just won't cut it. Regulatory agencies are calling for a more holistic approach—that's now possible with FMM, a particle-identifying technology exclusively available on Aura.
Now you can get 100% sampling efficiency at unparalleled speeds, making it possible to characterize and differentiate anything from a single aggregate protein or cell to tens of millions of particles in under two hours.
How FMM Works
The powerful combination of Fluorescence Membrane Microscopy (FMM) and Backgrounded Membrane Imaging (BMI) delivers an unmatched level of analysis to identify, count, and size particles that was never before possible.
In FMM, aggregates are labeled using specific fluorescent dyes or antibodies using flexible protocols that easily fit into your workflow, resulting in simple and definitive identification.
Actual FMM image of a heterogeneous particle sample
FMM Workflows
Solution Phase Stain
A fluorescent dye reagent is mixed with the sample in a test tube prior to applying it to a backgrounded plate. BMI and FMM measurements can then be taken.
Membrane Phase Stain
Microscopic particles are captured on the membrane surface and labeled with a fluorescent reagent. This can be accomplished using normal BMI steps with 40 µL of fluorescent reagent added to the sample with a second vacuum applied so the FMM measurement can be taken.
There are two methods for Aura immunoassay. One in which the antibody is applied in solution, the other while the sample is suspended on the membrane.
Featured products
Aura+
With the ability to support the full range of development and manufacturing applications across therapeutic approaches—including protein/antibody therapeutics, gene therapies, cell therapies, and small molecules— Aura+ packs powerful flexibility into a single particle analyzer.
Features
- 5 µL to 10 mL volume
- Particle characterization for protein, cell and gene therapeutics
- Three fluorescent channels for particle identification
- 4x and 20x magnification imaging
Aura GT
Optimized for gene therapy applications, Aura GT maximizes what you can learn about your particles while minimizing the amount of sample you need to analyze.
Features
- Ultra low volume requirement - 5 µL
- Specialized in viral vector stability analysis
- Fluorescence enabled for protein and nucleic acid analysis
- Gene therapy specific assays and workflows
