Do You Know What's Really in Your Sample?
Traditional fluidic imaging cytometry notoriously falls short while distinguishing between clusters of cells and other non-cellular material, leading to compromised results.This is only exacerbated by the difficulty of avoiding clogging and cross-contamination, further decreasing effectiveness.
It's evident that new technologies need to be developed to help bridge this gap and make accurate cell identification more efficient.
With Aura CL, you now have a solution.
Why Use Aura to Identify Cellular and Non-Cellular Particles?
- Definitively ID subvisible particles using fluorescent cell and other particle specific stains
- Obtain detailed information on particles that other methods can’t deliver, including size, morphology, count, and distribution
- Small to large cellular aggregate characterization in highly heterogeneous solutions
- 100x higher throughput compared to standard flow imaging and cytometry techniques for testing lots of formulations, conditions, and lot releases
- Fluidics-free rapid analysis time
- Evaluate a wide range of particle sizes, measuring 1 μm to 5 mm with high reproducibility
- Maintain compliance with the option for 21 CFR Part 11 software
Accurately Distinguish Between Cellular and Non-Cellular Particles
Aura CL stands out from other fluidic imaging cytometry techniques with its Fluorescent Membrane Microscopy (FMM) technology analyzing cells in the solid phase. For the first time, you can tell if your aggregate is cellular or non-cellular in your cell therapy, enabling you to develop a better understanding of quality attributes.
Aura CL delivers precise cellular particle count and characterization for superior quality assessment in the production process—so you get only the best therapeutic results.
Plus, state-of-the art features like fluidics-free prevent clogging issues seen in other methodologies. It enables high-throughput, high refractive index contrast, and 100% measurement efficiency for suspended cells, protein, and viral aggregates found in cell and gene therapies.
Make Better Decisions About Your Therapeutics
With Aura CL’s powerful, low-volume imaging capabilities, you can easily label cells in your samples with DNA-specific dyes and detect protein aggregates using Thioflavin T (ThT). Plus get unparalleled insight by combining this data with the distinct morphology information obtained via Backgrounded Membrane Imaging (BMI) and Side Illumination Membrane Imaging (SIMI) — all to reveal an unbiased view into every particle.
This comprehensive overview makes it easier than ever to make better decisions more quickly.
Accurately Monitor Cell Behavior
With Aura CL, you can easily determine if your therapeutic formulation or storage is the cause of cell aggregation.
Utilizing either DAPI or Hoechst staining to label nuclei and performing a comparison with BMI imaging gives an in-depth overview on whether singlets, doublets, triplets and beyond are present within your cellular population.
Particles can be identified through their fluorescent properties and overall area. See an example from each quadrant with singlets and doublets presenting a dual positive identification for DNA and protein based on the intensity of FL1 and FL2 measurements. Non-biological aggregates show negative for DNA and protein, while protein aggregates show particles positive for protein only.
Particle Vue Software
Get particle analysis answers in just a few clicks with flexible, easy-to-use Particle Vue Software.