Overcome Cell Viability Measurement Challenges
Flow cytometry and manual hemocytometry have long been the go-to methods for measuring cell viability and apoptosis. But their drawbacks—including clogging issues, sample wastage, labor intensity and slow speeds—can impede progress in critical research or production processes.
Distinguishing between cellular material from non-cellular particles is also difficult. Even with fluidics-based techniques, there is little contrast to help identify subvisible particles, impacting data accuracy along the way.
Because of this, it has been hard to find a fast, reliable, and high-throughput approach that can accurately measure cell viability and purity—but Aura CL is here to change that.
2D scatter plot of FL1 (ThioFlavin T) vs. FL2 (DAPI)
Determine the Success of Your Cell Therapy with Aura CL
With Aura CL, cell therapies are rigorously examined for quality and safety, ensuring they meet the specifications outlined by USP 1046.
Powered by Backgrounded Membrane Imaging (BMI) and Fluorescent Membrane Microscopy (FMM), this game-changing technology provides reliable indications of viable cells in your cell therapy product.
Add in Particle Vue software, and you get a speedy analysis on 96-well formats - allowing precise measurement of not only viability but also purity. Making sure that whether it's iPSCs or T-cells, you have all the information necessary with just one assay.
Identify Non-Viable and Viable Cells in Your Therapeutic
Aura CL's cell viability assay takes advantage of the increases in plasma membrane permeability observed during apoptosis to accurately identify and measure viable and non-viable cells.
After samples are incubated with a low concentration of a cell viability stain such as DAPI, only non-viable cells can be visualized due to the dye's impermeability. Utilizing Aura CL imaging technology, these labeled non-viable cells can be precisely quantified before applying an increased dose of the same staining agent in order to estimate and determine the amount of viable cell concentrations within each sample—producing results that rival traditional methods such as hemocytometry or flow cytometry.
Simplify Particle ID with FMM
Ensuring product quality and safety is paramount in the manufacturing process. However, using morphology alone to effectively identify particles with different root causes can be unreliable.
This is where FMM comes in. FMM offers an accurate solution by utilizing particle-specific dyes, like Thioflavin T (ThT), that bind to protein aggregates and simplify ID.
With FMM, you are able to easily distinguish between viable cells, non-viable cells, protein aggregates, and more – allowing for a reliable and efficient manufacturing process.
Featured products
Aura+
With the ability to support the full range of development and manufacturing applications across therapeutic approaches—including protein/antibody therapeutics, gene therapies, cell therapies, and small molecules— Aura+ packs powerful flexibility into a single particle analyzer.
Features
- 5 µL to 10 mL volume
- Particle characterization for protein, cell and gene therapeutics
- Three fluorescent channels for particle identification
- 4x and 20x magnification imaging
Aura CL
Safeguard your cell therapies from contamination with Aura CL. Our high-throughput, fluidic-free particle analysis system quickly and accurately identifies and characterizes subvisible particles, giving you peace of mind when it comes to cell therapy development and quality assessment.
Features
- Automated residual Dynabeads analysis
- Cell purity and viability characterization
- High powered magnification
- Fluidics free
