Free Fatty Acid Particle ID
Polysorbate degrades into lauric, myristic, and palmitic acid leading to the formation particles with distinct morphological characteristics. Lauric acid particles are large and irregular, myristic acid particles are small and oval-shaped, and palmitic acid particles are fibril-like.
While distinct, morphology is not an infallible method to identify the root causes of aggregation, especially in a complex sample. FMM can be used to confirm the identity of FFA particles. BODIPY™ FL C16 is a high-affinity stain that specifically labels FFA particles, distinguishing them from other particles in the sample.
Why Use Aura+ or Aura PTx to Monitor Polysorbate Degradation
- Complete biologic particle characterization for stability
- Definitive identification of free fatty acids, the degradation product of polysorbate
- Detailed information on particles – size, morphology, counts, distribution, identification
- Analysis with 5 µL to 10 mL of sample
- High-throughput 96-well format for testing lots of conditions
- Rapid analysis time of about 1 minute per sample
- High-resolution particle images
- Automated data analysis with Particle Vue software
- 21 CFR Part 11 compliant software upgrade available