Identify Non-viable and Viable Cells in Your Therapeutic
Aura CL’s cell viability assay uses the increase in plasma membrane permeability observed with apoptotic cells. Samples are first incubated with DAPI (4’6- diamidino-2 phenylindole) at a low concentration where the dye is cell impermeant to differentially label non-viable (dead) cells only. The labeled non-viable cells are imaged using Aura CL before the same samples are then labeled with a higher concentration of DAPI to label all the cells to determine the percentage of viable cells in the sample. Results are comparable to lower throughput methods like hemocytometry ad flow cytometry.
Simple, Accurate Particle ID
Accurate determination of subvisible particles (SVPs) and aggregates that can form during the manufacturing process is another key regulatory parameter required to ensure product quality and safety. However, using morphology alone to identify particles with different root causes is not reliable. FMM uses particle-specific dyes like Thioflavin T (ThT), which binds to protein aggregates, to simplify your ID – allowing you to accurately distinguish between viable cells, non-viable cells, protein aggregates, and non-cellular particulates.
Why Use Aura+ or Aura CL to Determine Cell Viability
- Accurate, fluidics-free analysis
- Definitive identification of cellular, protein, and non-biologic particles
- Provides detailed information on particles – size, morphology, counts, distribution, identification
- High-throughput 96-well format for testing lots of conditions
- Rapid analysis time of about 1 minute per sample
- Wide working range: Measure particles from 1 μm to 5 mm with high reproducibility
- Particles are imaged without the interference of buffer or matrix for higher sensitivity
- High-resolution particle images
- Automated data analysis
- 21 CFR Part 11 software available